Approximate Formula:
Ingredients
|
gm/liter
|
Sodium Glycerophosphate
|
10.0
|
Cysteine hydrochloride
|
0.5
|
Sodium Thioglycolate
|
0.5
|
Calcium chloride
|
0.1
|
Agar
|
5.0
|
Final pH 7.4±0.2 at 25 ℃
|
*Adjusted and/or supplemented as required to meet performance criteria.
|
Directions:
Suspend16.1gof the medium in one liter of distilled water or deionized water. Mix well and heat with frequent agitation. Sterilize in an autoclave at 121 ° C(15 lbs) for 15 minutes.
Principle and Interpretation:
The ability of Stuart transport medium to maintain the viability of gonococci during transport led other researchers to explore its use with a variety of specimens. This medium is currently recommended for throat, vaginal and wound samples.
This medium is chemically defined, semisolid, non-nutrient medium which prevent microbial proliferation. Because of its composition the medium ensures that microorganisms present are able to survive for a sufficiently long period of time. Calcium chloride along with sodium glycerophosphate acts as good buffering agent and also maintains osmotic equilibrium in the medium.
Appearance:
Dehydrated medium is a free-flowing white powder. The prepared medium is a kind of milky white transparent gel.
Precautions:
This medium is for laboratory use only. Dried medium which is past shelf life, caking or color variation cannot be used.
Storage conditions and Shelf life:
Stuart Transport Medium must be stored tightly capped in the original container at 5-30°C. The dehydrated medium has a shelf life of 3 years from date of manufacturing. Prepared medium may be stored, out of direct light at 2-8°C.
Quality control:
Cultural characteristics observed after incubation at 20 -25°Cfor 48 hours. Remove swabs, streak on prepared blood agar plates and incubate appropriately.
Microorganism
|
Strains Number
|
Growth
|
Remarks
|
Shigella flexneri
|
ATCC 12022
|
luxuriant
|
/
|
Salmonella typhimurium
|
ATCC 14028
|
luxuriant
|
/
|
Streptococcus pyogenes
|
ATCC 19615
|
luxuriant
|
/
|
Reference:
1. Stuart, 1946,GlasgowMed. J. 27:131.
2. Stuart, Toshach and Patsula, 1954,Can.J. Public Health, 45:73.
3. Ringertz, 1960, Acta Pathol. Microbiol. Scand., 48:105.
4. Maintenance of Medical Bacteria, Vol. I, Williams and Wilkins, Baltimore