Approximate Formula:
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Ingredients
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gm/liter
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|
Sodium thioglycolate
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1.5
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Sodium chloride
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5.0
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Disodium hydrogen phosphate
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1.1
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Calcium chloride
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0.09
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Agar
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5.0
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Final pH 8.4±0.2 at 25 ℃
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*Adjusted and/or supplemented as required to meet performance criteria.
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Directions:
1. Open the package before sampling.
2. Remove the sampling swab and transport tube.
3. Conduct sample collection at the specified sampling site.
4. Place the collected swab into the transport medium.
5. Mark Information of the sample
6. Transport to the laboratory and take out the sampling swab
7. Spread and isolate the sample
Precautions:
1. This product is only used for IVD.
2. Used medium should be disinfected.
3. Only opearted by the trained experimenters.
4. Wear gloves and mask to take protective measures.
5. Routine sample collection should be submitted within 24±2 hours, and the detection rate of bacteria will be affected if the sample is out of time.
6. Do not use after expiration.
Principle and Interpretation:
Cary-Blair Transport Medium is kind of medium used for transporting specimens of intestinal bacteria. The purpose of this medium is to maintain the viability of microbes during the time from collection to examine. This medium has a low oxidation/reduction potential, which assures bacterial survival for long periods of time
In the formulations, calcium chloride and sodium chloride provide essential ions that help maintain osmotic balance while controlling permeability of bacterial cells. Monopotassium phosphate and disodium phosphate provide buffering capabilities. Sodium thioglycollate suppresses oxidative changes and provides a reduced environment.
Storage conditions and Shelf life:
Cary-Blair Transport Medium must be stored tightly capped at 5-25°C. Cary-Blair Transport Medium Tube has a shelf life of 3 years from date of manufacturing. Must be stored out of direct light.
Quality control:
Cultural characteristics observed after incubation at 20 -25°C for 48 hours. Remove swabs, streak on prepared blood agar plates and incubate appropriately.
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Microorganism
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Strains Number
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Growth(Blood Agar Plates)
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Campylobacter jejuni
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ATCC 33291
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Luxuriant (42±1℃Micro-aerobic culture)
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Salmonella typhimurium
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ATCC 14028
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Luxuriant
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Vibrio parahaemolyticus
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ATCC 17802
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Luxuriant
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Shigella flexneri
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ATCC 12022
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Luxuriant
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Reference:
1.Collee J. G., Fraser A. G., Marmion B. P., Simmons A., (Eds.), Mackie and cCartney, Practical Medical Microbiology,1996, 14th Edition, Churchill Livingstone.
2.Pezzlo M., 1998, Clin. Microbiol. Rev., 1:268-280.
3.Wilkie M. E., Almond M. K., Marsh F. P., 1992, British Medical Journal 305:1137-1141.
Technical Data: