Approximate Formula:

Directions:

Suspend18.0gof the medium in one liter of distilled water or deionized water. Mix well and heat with frequent agitation. Sterilize in an autoclave at121℃(15 lbs.) for 15 minutes.

Principle and Interpretation:

Peptic digest of animal tissue and beef extract provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients necessary for the growth of bacteria. Sodium chloride maintains the osmotic equilibrium of the medium.

Appearance:

Dehydrated medium is a free-flowing yellowish granule.

Precautions:

This medium is for laboratory use only.

Dried medium which is past shelf life, caking or color variation cannot be used.

Storage conditions and Shelf life:

Nutrient Broth must be stored tightly capped in the original container at 5-30°C. The dehydrated medium has a shelf life of 3 years from date of manufacturing. Prepared medium may be stored, out of direct light at 2-8°C.

Quality control:

Inoculate and incubate at 36±1℃for 18-24 hours.

Reference：

1. GB4789.28-2013 People's Republic of China food safety national standard food microbiology test medium and reagent quality requirements.

2. Marshall (ed.). 1993. Standard methods for the examination of dairy products, 16th ed. American Public Health Association, Washington, D.C.

3. U.S. Food and Drug Administration. 2001. Bacteriological analytical manual, online. AOAC International, Gaithersburg, Md.

4. Downes and Ito (ed.). 2001. Compendium of methods for the microbiological examination of foods, 4th ed. American Public Health Association, Washington, D.C.