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MacConkey Agar Medium is used for selective isolation of intestinal bacteria.
Approximate Formula:
Components
Content(g/L)
Pancreatic digest of gelatin
17.0
Peptones (Meat and Casein)
3.0
Lactose Monohydrate
10.0
Sodium chloride
5.0
Bile salts
1.5
Agar
13.5
Neutral red
0.03
Crystal Violet
0.001
Final pH 7.1±0.2 at 25℃
*Adjusted and/or supplemented as required to meet performance criteria.
Directions:
Suspend 50.0g of the medium in 1L of distilled water,sterilize in an autoclave at 121 ℃ for 15 minutes.
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