Bismuth Sulfite Agar Medium is a medium used for Salmonella selective isolation.

Formula:

Pancreatic  Digest  of  Casein....5.0
Peptic  Digest  of  Animal  
Tissue........................................5.0
Beef  Extract..............................5.0
Ferrous  Sulfate.........................0.3
Bismuth  Sulfite  Indicator..........8.0
Disodium Hydrogen 
Phosphate.................................4.0
Dextrose....................................5.0       
Brilliant green........................0.025                           
Agar.........................................20.0           
Final pH7.6±0.2 at 25℃ 

Directions:

Suspend 50.3g of the medium in 1L of distilled water, after cooling to 50 ℃, pour into sterilized dishes. Do not overheat, need not autoclaving.

Quality Control and Typical Characteristics:

Salmonella is incubated at 37 ℃ 24-48 hours, the color of colonies is tan or gray to black, sometimes has a metallic sheen, medium around them is brown or black, some strains are grayish green, surrounded by the same medium or slightly darker.