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Shigella Chromogenic Medium

microbial medium
Chinese Name:志贺氏菌显色培养基
Product No.:HB7018
Specification:1000ml
Product Price:
Product Use:Specially used for the rapid detection of Shigella in food and patient stool samples. The colonies of Shigella are white or colorless, some sonnei Shigella have blue center
Remarks:

Technical Data:

Shigella Chromogenic Medium is specially used for the rapid detection of Shigella in food and patient stool samples. The colonies of Shigella are white or colorless, some sonnei Shigella have blue center; Escherichia coli and Coliforms have green or yellow colonies; salmonella are yellow or colorless and have a black center; other are blue-green or yellow. Proteus and Pseudomonas aeruginosa basically do not grow; it is an ideal culture medium for rapid detection.

Ingredients (g/L):

Special Nutrients

70.2

Chromogenic reagent

0.4

Agar

15.0

Final pH 7.3±0.1 at 25℃

*Adjusted and/or supplemented as required to meet performance criteria

Directions:

Suspend 17.12g of the medium in 200mL of distilled water, heat in 1 minute. When cooling to 45-50 ℃, pour into sterile petri dishes.

Warning: 

Prepared medium must be kept dry, can place inverted culture in incubator at 36 ± 1 ℃ for 1-2 hours.

Procedures:

1. Prepared sample solution according to national standards, SN standard, FDA standards or other methods.

2. Transfer 25g sample solution into 225mL GN Broth or Shigiella Broth, enrichment culture for 18- 24hours at 36 ± 1 ℃;

3. Take one ring enrichment broth with a 3mm inoculation loop, streak to inoculated o Shigella Chromogenic Medium, make two plates

4. Culture at 36 ± 1 ℃ for 20-24 hours, the colonies of Shigella are white or colorless, some sonnei Shigella have blue center; Escherichia coli and Coliforms have green or yellow colonies; salmonella are yellow or colorless and have a black center; other are blue-green or yellow. Proteus and Pseudomonas aeruginosa basically do not grow

5.  For typical colonies, do Shigella serological test and full set of biochemical tests.

Quality Control:

1. Appearance:

The dehydrate powder medium is homogeneous, having good flowability, grey white to pale pink, prepared medium is red solid.

2. Microbial sensitivity test:

Incubated at 36 ± 1 ℃ 18-24h:

QC Strains

Strains No.

Growth

Colonies color

Shigella flexneri

ATCC12022

+++

Colorless or white, 

the diameter of colonies is 1-3mm

Shigella dysentery

CMCC51252

+++

Colorless or white,

 the diameter of colonies is 1-3mm

Shigella boydii

CMCC51582

+++

Colorless or white, 

the diameter of colonies is 1-3mm

sonnei Shigella

CMCC51592

+++

Colorless or white, the diameter of 

colonies is 1-3mm, with blue center

Staphylococcus aureus

ATCC25923

-

/

E. coli

ATCC25922

+++

green

salmonella

ATCC14028

+++

Colorless with black center

Proteus mirabilis

ATCC35029

-/+

Colorless, diameter<1mm

Klebsiella pneumoniae

ATCC10031

++

Colorless with black center

Freundii Citrobacter

ATCC43864

+++

Colorless with black center

Pseudomonas aeruginosa

CICC10211

-/+

Colorless, diameter<1mm

Methods limitations:

The typical Shigella on t medium must be do serological test and biochemical tests

Typical features:

The colonies of Shigella are white or colorless, some sonnei Shigella have blue center; Escherichia coli and Coliforms have green or yellow colonies; salmonella are yellow or colorless and have a black center; other are blue-green or yellow. Proteus and Pseudomonas aeruginosa basically do not grow.

Storage:

The prepared plates can be kept for 2-5 days, avoid direct exposure to light. The powder medium should be placed in a dark, dry place, storage temperature 2-8 ℃, avoid direct exposure to light.

Failure:

Dry powder media which are over the shelf life, caking and color variation cannot be used.

Note:

This medium is only for laboratory use.

Reference:

1. Restaino, L., E. W. Frampton, W. C. Lionberg, and A. L. Restaino. 2007. Detection of Shigella sp.using a selective and differential chromogenic plating medium. Abstract P3-43. IAFP.


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