Candida Chromogenic Medium is used for the isolation and identification of Candida, the colonies of Candida albicans are blue-green. Candida chromogenic medium based on enzyme substrate technique, distinguish different Candida by colors, incubated at 25-28 ℃ for 48 hours, the colonies of Candida albicans are blue-green, Candida tropicalis are blue-gray or blue, Candida krusei are pink, other Candida are inhibited.

Ingredients (g/L):

*Adjusted and/or supplemented as required to meet performance criteria.

Directions:

Suspend 9.6g of the medium in 200mL of distilled water (adjust by proportion 4.8g/100mL), boil in one minute. Cooling the medium to 45-50 ℃ by in a water bath, , pour into sterile petri dishes (15ml for 9cm dish, 10mL for 7cm dish), after agar completely solidifying, put in incubator inverted at 36 ℃ ± 1 ℃ for 0.5-1 hours.

Procedures:

1. Prepare the sample solution according to national standards, SN standard, FDA standards or other methods;

2. Take the sample solution and apply or streak it evenly on the plate;

3. Incubate at 25-28 ℃ for 48h. the colonies of Candida albicans are blue-green, Candida tropicalis are blue or blue-gray, Candida glabrata are purple, Candida krusei are pink, other Candida are inhibited.

4. Undetermined Candida albicans colonies can streak onto PDA agar plates, at 25-28 ℃ for 24-48 hours, pick single colony to do microscopy and biochemical tests.

Quality Control:

1. Appearance:

The dehydrate powder medium is homogeneous, having good flow ability; milky white, prepared medium is colorless solid.

2. Microbial sensitivity test:

Incubated at 36 ± 1℃ 18-24h:

Typical features:

The typical colonies of Candida albicans are blue-green, Candida tropicalis are blue or blue-gray, Candida glabrata are purple, Candida krusei are pink, other Candida are inhibited.

Storage:

The prepared plates must be used immediately, avoid direct exposure to light. 

The powder medium should be placed in a dark, dry place, storage temperature 2-8 ℃, avoid direct exposure to light.

Failure:

Dry powder media which are over the shelf life, caking and color variation cannot be used.

Note:

This medium is only for laboratory use.

Reference:

1. Mega et al (J. Biochem. vol. 71, pp 107-114, 1972). 
2. Bendel et al. XP-002064132 "Distinct Mechanisms of Epithelial Adhesion for Candida albicans and Candida tropicalis" vol. 92, Oct. 1993, pp. 1840-1849. 
3. Mira-Gutierrez et al., XP-002064133 "Identification of yeasts by hydrolysis of amides" Nov. 8, 1994, pp. 101-106. Crist, Jr. et al., XP-002064134 "Comparison of the MUREX C. Albicans, Albicans/Sure, and Bacticard Candida Test Kits with the Germ Tube Test for Presumptive identification of Candida albicans" vol. 36, Jul. 5, 1996, pp. 2616-2618.