Enterococcus Chromogenic Medium is used for rapid detection of food, water and environment, 

Enterococcus is red to purple colonies.

Ingredients (g/L):

*Adjusted and/or supplemented as required to meet performance criteria

Directions:

Suspend 56.4g of the medium in 1L of distilled water, dissolved by a water bath, when cooling to 45-50 ℃, poured into sterile petri dishes. Need not autoclaving.

Procedures:

Before to use, the prepare medium is placed at room temperature for 10-20 minutes

1. Prepared sample solution according to national standards, SN standard, FDA standards or other methods..

2. Transfer 1ml sample solution into Enterococcus Chromogenic Medium which cooling to 45-50 ℃, mix thoroughly or coat on plate, or take sample solution streak on Enterococcus Chromogenic Medium.

3. Culture at 36 ± 1 ℃ for 24-48h, select 30-200 colonies, count red to purple colonies on plates.

Quality Control:

1. Appearance:

The dehydrate powder medium is homogeneous, having good flowability, yellowish or milky white, prepared medium is yellowish solid.

2. Microbial sensitivity test:

Incubated at 36 ± 1 ℃ 18-24h:

Typical features:

The colonies of Enterococcus are red to purple.

Storage:

The prepared plates must be used immediately, avoid direct exposure to light. 

The powder medium should be placed in a dark, dry place, storage temperature 2-8℃, avoid direct exposure to light.

Failure:

Dry powder media which are over the shelf life, caking and color variation cannot be used.

Note:

This medium is only for laboratory use.