Urine Orientation Chromogenic Medium (UTI) is a chromogenic medium for the presumptive

identification and differentiation of all the main micro-organisms that cause urinary tract infections 

Formula:

Directions:
Suspend 34.5 grams of Urine Orientation Chromogenic Medium (HB7013) in 1 litre of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and pour into sterile Petri dishes.

Description:
Urine Orientation Chromogenic Medium contains two specific chromogenic substrates which are cleaved by enzymes produced byEnterococcus spp., E. coli and coliforms. In addition, it contains tryptophan which indicates tryptophan deaminase activity (TDA), indicating the presence of Proteus spp. It is based on Cystine Lactose Electrolyte Deficient (CLED) Medium which provides a valuable non-inhibitory diagnostic agar for plate culture of other urinary organisms, whilst preventing the swarming of Proteus spp.
The chromogen, X-glucoside, is targeted towards ß-glucosidase enzyme activity, and allows the specific detection of enterococci through the formation of blue colonies.
The other chromogen, Red-Galactoside, is cleaved by the enzyme ß-galactosidase which is produced by E. coli, resulting in pink colonies. Any uncertainty in identification may be resolved by removing suspect colonies from the plate and performing an indole test using Kovacs Indole Reagents (code HB8279).
The medium also contains tryptophan which acts as an indicator of tryptophan deaminase activity (TDA), resulting in halos around the colonies of Proteus, Morganella and Providencia spp.

Appearance:
Dehydrated Culture Medium is a free-flowing straw -colored powder. 
The prepared medium is a straw-colored, transparent agar.
Precautions:
Urine Orientation Chromogenic Medium should only be used for in vitro diagnostic purposes. 

Do not use beyond the stated expiry date, or if the product shows any sign of deterioration.

Storage conditions and Shelf life:
Urine Orientation Chromogenic Medium must be stored tightly capped in the original container at 10-30°C. 

The dehydrated medium has a shelf life of 3 years from date of manufacture. 

Prepared medium may be stored, out of direct light for up to 2 weeks at 2-8°C.

Quality control:

Test Organisms	Incubation			Results
	Time	Temperature	Atmosphere
Escherichia coli ATCC® 25922	24hr	36°C±1°C	Aerobic	Growth;  medium sized rose to magenta colonies with darker pink centers
Klebsiella pneumoniae ATCC® 13883	24hr	36°C±1°C	Aerobic	Growth; large, deep blue or dark indigo colonies
Enterococcus faecalis ATCC® 29212	24hr	36°C±1°C	Aerobic	Growth; small, teal to turquoise colonies
Proteus mirabilis ATCC® 12453	24hr	36°C±1°C	Aerobic	Growth; clear to light yellow colonies with golden-orangecolor diffused  through surrounding media
Staphylococcus aureus ATCC® 25923	24hr	36°C±1°C	Aerobic	Growth; opaque, cream to  white colored colonies
Pseudomonas aeruginosa ATCC® 27853	24hr	36°C±1°C	Aerobic	Growth; colorless to light yellow-green, translucentcolonies, which may have a slight iridescence
Staphylococcus  saprophyticus ATCC® 15305	24hr	36°C±1°C	Aerobic	Growth; opaque, pink colonies
Candida albicans ATCC® 10231	24hr	36°C±1°C	Aerobic	Growth; small, white,moist colonies
Citrobacter freundii ATCC® 8090	24hr	36°C±1°C	Aerobic	Growth;  dark blue colonies, often with  a rose halo in the surrounding media

Reference:

1.Collee J. G., Fraser A. G., Marmion B. P., Simmons A., (Eds.), Mackie and cCartney, Practical Medical Microbiology,1996, 14th Edition, Churchill Livingstone.

2.Pezzlo M., 1998, Clin. Microbiol. Rev., 1:268-280.

3.Wilkie M. E., Almond M. K., Marsh F. P., 1992, British Medical Journal 305:1137-1141.

4.Friedman M. P. et al, 1991, J. Clin. Microbiol., 29:2385-2389.

5.MurrayP., Traynor P. Hopson D., 1992, J. Clin. Microbiol., 30:1600-1601.

6.Soriano F., Ponte C., 1992, J. Clin. Microbiol., 30:3033-3034.

7.Merlino et al, 1995, Abstr. Austr. Microbiol. 16(4):17-3.