Staphylococcus aureus Chromogenic Medium is a used according to enzyme substrate method, mold and yeast chromogenic medium distinguish between different molds and yeasts by color, after culture for 3-5 days at 25-28 ℃, the colonies of Candida albicans are blue-green, Candida tropicalis are blue-green, Candida glabrata are blue-green, Candida krusei are blue-green, Saccharomyces cerevisiae are blue-green, blue-green mold are cotton-like blue-green, bacteria are suppressed.

Ingredients (g/L):

*Adjusted and/or supplemented as required to meet performance criteria.

Instructions:

Suspend 9.5g of the medium in 200mL of distilled water(according to the proportion of 4.75g/100mL to adjust), boil in one minute. After cooling to 45-50 ℃in a water bath, gently shake and pour into sterile petri dishes (15ml for 9cm dish, 10ml for 7cm dish), after agar completely solidification, inverted culture in incubator at 36 ℃ ± 1 ℃ for 0.5-1.

Procedures:

1. Prepared sample solution according to national standards, SN standard, FDA standards or other methods.

2. Take sample solution, coat or streaked on the plates

3. culture for 3-5 days at 25-28 ℃, the colonies of Candida albicans are blue-green, Candida tropicalis are blue-green, Candida glabrata are blue-green, Candida krusei are blue-green, Saccharomyces cerevisiae are blue-green, blue-green mold are cotton-like blue-green, bacteria are suppressed.

4. For undermined colonies of Candida albicans can be streaked onto PDA agar plates, 25-28 ℃ for 24-48 hours, select single colony to do microscopy and biochemical tests

Quality Control:

1. Appearance:

The dehydrate powder medium is homogeneous, having good flowability, milky white, prepared medium is colorless solid.

2. Microbial sensitivity test:

Incubated at 36 ± 1 ℃ 18-24h:

Methods limitations:

The medium identification of mold and yeast, may occur false-positive in a few cases, but no false-negative, sometimes it needs to do other complementary tests

Typical features:

The colonies of Candida albicans are blue-green, Candida tropicalis are blue-green, Candida glabrata are blue-green, Candida krusei are blue-green, Saccharomyces cerevisiae are blue-green, blue-green mold are cotton-like blue-green, bacteria are suppressed.

Storage:

The prepared plates must be used immediately, avoid direct exposure to light. The powder medium should be placed in a dark, dry place, storage temperature 2-8 ℃, avoid direct exposure to light.

Failure:

Dry powder media which are over the shelf life, caking and color variation cannot be used.

Note:

This medium is only for laboratory use.

Reference:

1. Mega et al (J. Biochem. vol. 71, pp 107-114, 1972).

2. Bendel et al. XP-002064132 "Distinct Mechanisms of Epithelial Adhesion for Candida albicans and Candida tropicalis" vol. 92, Oct. 1993, pp. 1840-1849.

3.Mira-Gutierrez et al., XP-002064133 "Identification of yeasts by hydrolysis of amides" Nov. 8, 1994, pp. 101-106.

Crist, Jr. et al., XP-002064134 "Comparison of the MUREX C. Albicans, Albicans/Sure, and Bacticard Candida Test Kits with the Germ Tube Test for Presumptive identification of Candida albicans" vol. 36, Jul. 5, 1996, pp. 2616-2618.