Total Genes Chromogenic Medium is a medium used for fast, accurate detection of total bacterial count in food, water and the environment, culture for 24 hours, bacterial colonies are red.

Ingredients (g/L):

*Adjusted and/or supplemented as required to meet performance criteria.

Directions:

Suspend 23.5g of the medium in 1L of distilled water, dispensing into flasks, at 121℃ autoclaving for 15 minutes.

Pouring method:

Take 1mL prepared sample of suitable concentration into a sterile petri dish, transfer 15mL chromogenic medium which cooling down to 45-50 ℃, mix, inverted culture at 36 ± 1 ℃ for 18-24h after solidification

Streaking method:

When cooling down to 45-50 ℃, pour into a sterile petri dish, after complete solidification, inverted culture at 37 ℃ in incubator for 1-2 hours, add 1mL liquid sample with a suitable concentration, streak onto the medium , culture at 36 ± 1 ℃ for 18-24h.

Procedures:

1. Prepared sample solution according to national standards, SN standard, FDA standards or other methods.

2. Transfer 1mL sample onto chromogenic medium cooling down to 45-50 ℃, mix thoroughly or coating on plate;

3. Culture at 36 ± 1 ℃ for 18-24h, select the plate which have 30 to 200 typical colonies of E. coli, total count of the red colonies on the plate (Regardless of size and light or deep color)

Quality Control:

1. Appearance:

The dehydrate powder medium is homogeneous, having good flowability, milky white, prepared medium is colorless solid.

2. Microbial sensitivity test:

Incubated at 36 ± 1 ℃ 18-24h:

Methods limitations:

When the medium is used for total count, some colonies are colorless, but they are must counted in total genes.

Typical features:

Typical colonies are red to light red.

Storage:

The prepared plates must be used immediately, avoid direct exposure to light. The powder medium should be placed in a dark, dry place, storage temperature 2-8 ℃, avoid direct exposure to light.

Failure:

Dry powder media which are over the shelf life, caking and color variation cannot be used.

Note:

This medium is only for laboratory use.