7. Peotone Glucose Yeast extract Medium PGY (蛋白胨、酵母膏、葡萄糖培养基)
Peptone(蛋白胨) 10g Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 1g Distilled water (蒸馏水) 1L
8. Glycerol Agar (甘油琼脂)
Peptone (蛋白胨) 5g Beef extract (酵母膏) 3g
Glycerol (甘油) 20g Top water (自来水) 1000ml
Agar (琼脂) 15g pH 7.0-7.2
9. Rhizobium medium (根瘤菌培养基)AS 9
Yeast eztract (酵母膏) 1g Soil eztract (土壤浸提液) 200ml
Mannitol (甘露醇) 10g Agar (琼脂) 15g
Distilled water (蒸馏水) 800ml pH 7.2
[Note]:Soil extract:Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃ , then mixed with othringredients and distributed. (土壤浸提液的制法:取土壤50克,加水200毫升,15磅蒸煮1小时,经滤纸过滤后加水补足到200毫升。)
适用范围:大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、
大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌
10. Mannitol Agar (甘露醇琼脂)
Yeast extract (酵母膏) 5g Peptone (蛋白胨) 3g
Mannitol (甘露醇) 25g Agar (琼脂) 15g
Distilled water (蒸馏水) 1000ml
11. Glucose Asparagine (葡萄糖、天门冬素琼脂)
Glucose (葡萄糖) 10g Asparagine (天门冬素) 0.5g
K2HPO4 0.5g Water (水) 1000ml
pH 7.2-7.4
适用范围:刺孢小单孢菌绛红变种、紫色小单孢菌(绛红小单孢菌)
12. Gause′s Synthetic Agar (高氏合成一号琼脂)
KNO3 1g Soluble starch(可溶性淀粉) 20g
K2HPO4 0.5g MgSO4.7H2O 0.5g
NaCl 0.5g FeSO4 0.01g
Agar (琼脂) 20g water (水) 1000ml
pH 7.2-7.4
适用范围:刺孢小单孢菌绛红变种、紫色小单孢菌(绛红小单孢菌)、白黄链霉菌、白色链霉菌、抗生链霉菌、双重轮丝链霉菌、产色链霉菌、烬灰链霉菌、天蓝色链霉菌、灭蚊链霉菌、红霉素链霉菌、青色链霉菌、球孢链霉菌、浅灰链霉菌、灰色链霉菌、吸水链霉菌、淡紫灰链霉菌、黄色长孢链霉菌、藤黄色链霉菌、细黄链霉菌、黑化链霉菌、玫瑰色链霉菌、华美链霉菌、嗜热链霉菌、委内瑞拉链霉菌、紫色直丝链霉菌、紫色链霉菌、绿色链霉菌
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